Chemiluminescent assay for the quantitative determination of -galactosidase activity in transfected cells

Equipment and additionally required solutions The -Gal Reporter Gene Assay can be performed in all automated or manual luminometers in tube and microplate format, as well as in scintillation counters. When using the microplate format, black or white microplates must be used. Except for redist. water (required for reconstitution and dilution purposes), the kit contains all assay reagents for lysing cells and determining -galactosidase activity. For standard cell washing steps, phosphate buffered saline (PBS) is also required.